Micropropagation of Shorea roxburghii G.Don by shoot apex culture
| Publication Type | Journal Article |
| Year of Publication | 2000 |
| Authors | Nakamura, K,; Soda, R,; Ide, Y, |
| Journal Title | Bio-technology applications for reforestation and biodiversity conservation. Proceedings of the 8th International Workshop of BIO-REFOR, Kathmandu, Nepal, November 28-December 2, 1999 |
| Pagination | 105-108 |
| Key Words | afforestation; biotechnology; micropropagation; techniques; tissue culture; Shorea roxburghii |
| Abstract | Shoot apices of Shorea roxburghii were cultured in a liquid MS medium containing zeatin with horizontal rotation culture. After 3 months of culture, multiple shoots were formed. To elongate shoots from multiple shoots, the multiple shoots were cultured in the liquid medium containing zeatin with rotary culture. Shoots were excised from multiple shoots and planted in the Florialite supplemented with 1/2 MS liquid medium. The shoots rooted well after one month of culture. Rooting rate was 98%. Plantlets were transplanted into pots in a green house for the acclimatization. The survival rate of plantlets was 98%. |
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